Indholdsfortegnelse Instituttets årsberetning - forskning - forbrug Publikationerne kan søges i Den Danske Forskningsdatabase

INSTITUT FOR MOLEKYLÆR OG STRUKTUREL BIOLOGI

• Amtoft, H.W.; Sørensen, A.B.; Bareil, C.; Schmidt, J.; Luz, A.; Pedersen, F.S.: Stability of AML1 (core) site enhancer mutations in T-lymphomas induced by attenuated SL3-3 murine leukemia virus mutants. J. Virol. ; 71: 5080-5087, 1997.
• Andersen, J.L.; Garrett, R.A.; Kjems, J.: Mapping the catalytic sites of two homing endonucleases. EMBO J ; 16: 3272-3281, 1997.
• Andersen, L.B.; Lund, A.; Kveiborg, M.; Clark, B.F.C.; Rattan, S.I.S.: From genes to functional gene products during ageing. In: Molecular Gerontology. Research Status and Strategies . Rattan, S.I.S.; Toussaint, O. (Eds.). Plenum Press, New York: 53-73, 1997.
• Andersen, M.H.; Berglund, L.; Rasmussen, J.T.; Petersen, T.E.: Bovine PAS-6/7 binds α _v β ₅ integrin and anionic phospholipids through two domains. Biochemistry ; 18: 5441-5446, 1997.
• Andreasen, P.A.; Kjøller, L.; Christensen, L.; Duffy, M.J.: The urokinase-type plasminogen activator system in cancer metastasis. A Review. Int.J. Cancer ; 72: 1-22, 1997.
• Barciszewski, J.; Siboska, G.E.; Pedersen, B.O.; Clark, B.F.C.; Rattan, S.I.S.: A mechanism for the in vivo formation of N ⁶ -furfuryladenine, kinetin, as a secondary oxidative damage product of DNA. FEBS Lett. ; 414: 457-460, 1997.
• Borg, S.; Brandstrup, B.; Jensen, T.J.; Poulsen, C.: Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus , and expression of corresponding mRNAs in developing root nodules. Plant J ; 11: 237-250, 1997.
• Cai, D.; Kleine, M.; Kifle, S.; Harlof, H.J.; Sandal, N.N.; Marcker, K.A.; Klein-Langhorst, R.M.; Salentijn, E.M.J.; Lange, W.; Stiekema, W.J.; Wyss, U.; Grundler, F.M.W.; Jung, C.: Positional cloning of a gene for nematode resistance in sugar beet. Science ; 275: 832-834, 1997.
• Clark, B.F.C.; Nyborg, J.: The ternary complex of EF-Tu and its role in protein biosynthesis. Curr. Opinion Struc. Biol. ; 7: 110-116, 1997.
• Damgaard, O.; Jensen, L.H.; Rasmussen, O.S.: Agrobacterium tumefaciens -mediated transformation of Brassica napus winter cultivars. Transgenic Res. ; 6: 279-288, 1997.
• Egelund, R.; Schousboe, S.L.; Sottrup-Jensen, L.; Rodenburg, K.W.; Andreasen, P.A.: Type-1 plasminogen activator inhibitor. Conformational differences between latent, active, reactive centre-cleaved, and plasminogen activator-complexed forms, as probed by proteolytic susceptibility. Eur.J. Biochem. ; 248: 775-785, 1997.
• Ellgaard, L.; Holtet, T.L.; Nielsen, P.R.; Etzerodt, M.; Gliemann, J.; Thøgersen, H.C.: Dissection of the domain architecture of RAP, the α ₂ -macroglobulin receptor associated protein. Eur.J. Biochem. ; 244: 544-551, 1997.
• Ethelberg, S.; Hallberg, B.; Lovmand, J.; Schmidt, J.; Luz, A.; Grundström, T.; Pedersen, F.S.: Second site proviral enhancer alterations induced by enhancer mutants of SL3-3 murine leukemia virus: Negative effect of nuclear factor 1 binding site. J. Virol. ; 71: 1196-1206, 1997.
• Ethelberg, S.; Lovmand, J.; Schmidt, J.; Luz, A.; Pedersen, F.S.: Increased lymphomagenicity and restored disease specificity of AML1 site (core) mutant SL3-3 murine leukemia virus by a second site enhancer variant evolved in vivo . J. Virol. ; 71: 7273-7280, 1997.
• Ethelberg, S.; Sørensen, A.B.; Schmidt, J.; Luz, A.; Pedersen, F.S.: An SL3-3 murine leukemia virus enhancer variant more pathogenic than the wild type obtained by assisted molecular evolution in vivo . J. Virol. ; 71: 9796-9799, 1997.
• Heegaard, C.W.; Andreasen, P.A.; Petersen, T.E.; Rasmussen, L.K.: Binding of plasminogen and tissue-type plasminogen activator to dimeric α _S2 -casein accelerates plasmin generation. Fibrinolysis and Proteolysis ; 11: 29-36, 1997.
• Heegaard, C.W.; Larsen, L.B.; Rasmussen, L.K.; Højberg, K.- E.; Petersen, T.E.; Andreasen, P.A.: The plasminogen activation system in human milk. J. Pediatr. Gastroenterol Nutr. ; 25: 159-166, 1997.
• Hjorth, J.P.: Kloning af får - og mennesker? Ugeskrift for læger ; 159: 4741, 1997.
• Hjorth-Sørensen, B.; Pallisgaard, N.; Grönholm, M.; Hokland, P.; Clausen, N.; Jørgensen, P.: A novel MLL-AF10 fusion gene detected in an acute myeloid leukemia patient using a newly developed asymmetric reverse transcription PCR method. Leukemia ; 11: 1588-1593, 1997.
• Holst-Hansen, P.; Palacios Moreno, J.M.; Kildsgaard, J.; MacDougall, J.; Egebjerg, J.; Mortensen, K.K.; Sperling-Petersen, H.U.: Immunochemical determination of the cellular content of polypeptide chain release factor RF3 in Escherichia coli . Biochimie ; 79: 725-729, 1997.
• Holtet, T.L.; Graversen, J.H.; Clemmensen, I.; Thøgersen, H.C.; Etzerodt, M.: Tetranectin, a trimeric plasminogen-binding C-type lectin. Prot. Science ; 6: 1511-1515, 1997.
• Haaning, J.; Oxvig, C.; Overgaard, M.T.; Sottrup-Jensen, L.: Simple and reliable procedure for PCR amplification with short sequencing primers of genomic DNA from yeast. Biochem. Mol. Biol. Internat. ; 42: 169-172, 1997.
• Jensen, T.H.; Jensen, A.; Kjems, J.: Probing the structure of HIV-1 Rev by protein footprinting of multiple monoclonal antibody-binding sites. FEBS Lett. ; 414: 50-54, 1997.
• Jensen, T.J.; Borg, S.; Poulsen, C.: Small GTP-binding proteins in Lotus japonicus . In: Biological Nitrogen Fixation for the 21st Centure . Elmerich, C.; Kondorosi, A.; Newton, W.E. (Eds.). Kluwer Academic Publishers Dordrecht-Boston-London: 231-232, 1997.
• Jespersen, T.; Duch, M.; Pedersen, F.S.: Efficient non-PCR overlap extension of PCR fragments by exonuclease end-polishing. BioTechniques ; 23: 48-52, 1997.
• Jones, T.A.; Kjeldgaard, M.: Electron Density Map Interpretation. Meth. Enz. ; 277: 173-208, 1997.
• Kastrup, J.S.; Rasmussen, H.; Nielsen, B.B.; Larsen, I.K.; Holtet, T.L.; Graversen, J.H.; Etzerodt, M.; Thøgersen, H.C.: The human plasminogen binding protein tetranection: Crystallization and preliminary X-ray analysis of the C-type lectin domain and the full length protein. Acta Cryst. D ; 53: 108-111, 1997.
• Kasza, A.; Petersen, H.H.; Heegaard, C.W.; Oka, K.; Christensen, A.; Dubin, A.; Chan, L.; Andreasen, P.A.: Specificity of serine proteinase/serpin complex binding to very low density lipoprotein receptor and α ₂ -macroglobulin receptor/LDL-receptor-related protein. Eur.J. Biochem. ; 248: 270-281, 1997.
• Kjøller, L.; Kanse, S.; Kirkegaard, T.; Rodenburg, K.W.; Rønne, E.; Goodmann, S.L.; Preissner, K.T.; Ossowski, L.; Andreasen, P.A.: Plasminogen activator inhibitor-1 represses integrin- and vitronectin-mediated cell migration independently of its function as an inhibitor of plasminogen activation. Exp. Cell Res. ; 232: 420-429, 1997.
• Knudsen, B.R.; Dahlstrøm, K.; Westergaard, O.; Jayaram, M.: The yeast site-specific recombinase Flp mediates alcoholysis and hydrolysis of the strand cleavage product: Mimicking the strand joining reaction with non-DNA nucleophiles. J. Mol. Biol. ; 266: 93-107, 1997.
• Knudsen, C.R.: Hvad kan protein engineering bruges til? Struktur- funktionsanalyse af elongeringfaktor Tu ved anvendelse af protein engineering. Carlsbergfondet årsskrift ; 10-17, 1997.
• Kristensen, T.; Rasmussen, T.E.; Pedraza-Díaz, S.; Laustsen, P.G.: The complete amino acid sequence of human placental amino peptidase. FASEB J ; 11: A1307, 1997.
• Krusell, L.; Rasmussen, I.; Gausing, K.: DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley ( Hordeum vulgare ). FEBS Lett. ; 408: 25-29, 1997.
• Laursen, J.; Hjorth, J.P.: A cassette for high-level expression in the mouse salivary glands. Gene ; 198: 367-372, 1997.
• Laustsen, P.G.; Rasmussen, T.E.; Petersen, K.; Pedraza-Diaz, S.; Moestrup, S.; Gliemann, J.; Sottrup-Jensen, L.; Kristensen, T.: The complete amino acid sequence of human placental oxytocinase. Biochim. Biophys. Acta ; 1352: 1-7, 1997.
• Liang, Z.; Sottrup-Jensen, L.; Aspan, A.; Hall, M.; Söderhäll, K.: Pacifastin, a novel 155 kDa proteinase inhibitor from crayfish plasma, contains a unique three-domain transferrin chain. Proc. Natl. Acad. USA ; 94: 6682-6687, 1997.
• Lund, A.H.; Duch, M.; Lovmand, J.; Jørgensen, P.; Pedersen, F.S.: Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer. J. Virol. ; 71: 1191-1195, 1997.
• MacDougall, J.; Holst-Hansen, P.; Mortensen, K.K.; Freistroffer, D.V.; Pavlov, M.Y.; Ehrenberg M.; Buckingham, R.H.: Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system. Biochimie ; 79: 243-246, 1997.
• Mansilla, F.; Knudsen, C.R.; Laurberg, M.; Clark, B.F.C.: Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region. Protein Eng. ; 10: 927-934, 1997.
• Martensen, P.M.; Oka, K.; Christensen, L.; Rettenberger, P.M.; Petersen, H.H.; Christensen, A.; Chan, L.; Heegaard, C.W.; Andreasen, P.A.: Breast carcinoma epithelial cells express a very low density lipoprotein receptor variant lacking the O-linked glycosylation domain encoded by exon 16, but with full binding activity for serine proteinase/serpin complexes and M _r 40,000 receptor-associated-protein. Eur.J. Biochem. ; 248: 583-591, 1997.
• Meyer, K.N.; Kjeldsen, E.; Straub, T.; Knudsen, B.R.; Hickson, I.D.; Kikuchi, A.; Kreipe, H.; Boege, F.: Cell-cycle coupled relocation of types I and II topoisomerases and modulation of catalytic enzyme activities. J. Cell. Biol. ; 136: 775-788, 1997.
• Mortensen, K.K.; Palacios, J.M.; Holst-Hansen, P.; Steffensen, S.; Buckingham, R.; Sperling-Petersen, H.U.: Studies of E.coli translation termination factor RF3. FASEB J ; 11, A1404: 3205, 1997.
• Mortensen, K.K.; Palacios, J.M.; Steffensen, S.; Buckingham, R.; Sperling-Petersen, H.U.: Studies of wild type and recombinant E.coli translation termination factor RF3. 17th International tRNA workshop, maj 1997. Ciba, Japan, 1997.
• Nielsen, B.B.; Kastrup, J.S.; Rasmussen, H.; Holtet, T.L.; Graversen, J.H.; Etzerodt, M.; Thøgersen, H.C.; Larsen, I.K: Crystal structure of tetranectin, a trimeric plasminogen-binding protein with an alfa-helical coiled coil. FEBS Lett. ; 412: 388-396, 1997.
• Nielsen, P.K.; Haaning, J.; Sottrup-Jensen, L.: Characterization of bovine structure-specific recognition protein 1 (SSRP1) cDNA. Biochem. Mol. Biol. Internat. ; 43: 781-786, 1997.
• Nielsen, P.K.; Larsen, T.; Hønberg, L.S.; Sperling-Petersen, H.U.; Sottrup-Jensen, L.: Production and characterization of monoclonal and polyclo nal antibodies against bovine osteocalcin. Biochem. Mol. Biol. Internat. ; 42: 749-757, 1997.
• Nielsen, P.R.; Ellgaard, L.; Etzerodt, M.; Thøgersen, H.C.; Poulsen, F.M.: Structure of the N-terminal domain of α ₂ -macroglobulin receptor associated protein. Proc. Natl. Acad. Sci. USA ; 94: 7521-7525, 1997.
• Nolsøe, S.; Thirup, S.; Etzerodt, M.; Thøgersen, H.C.; Nyborg, J.: Crystallisation and preliminary X-ray diffraction studies of psoriasin. Acta Cryst. D ; 53: 119-121, 1997.
• Nyborg, J.; Nissen, P.; Kjeldgaard, M.; Thirup, S.; Polekhina, G.; Clark, B.F.C.: Macromolecular mimicry in protein biosynthesis. Folding & Design ; København, Danmark, 2: S7-S11, 1997.
• Olesen, M.R.; Jochimsen, B.U.: Identification of enzymes involved in indole-3-acetic acid degradation. In: Current Issues in Symbiotic Nitrogen Fixation . Elkan, G.H.; Upchurch, R.G. (Eds.). Kluwer Academic Publishers, Dordrecht: 143-149, 1997.
• Palacios, J.M.; Kildsgaard, J.; Mortensen, K.K.; Sperling-Petersen, H.U.: Functional studies on E.coli IF2 C-terminal deletion mutants and maps as helping tools. Human Capital and Mobility Network on Molecular Mechanism of Initiation of Prokaryptic Translation and Molecular Mechanism of Elongation Factor Tu Joint Annual Meeting, maj 1997. Island of Spetses, Greece, 1997.
• Pedersen, L.; van Zeijl, M.; Johann, S.V.; O'Hara, B.: Fungal phosphate transporter serves as a receptor backbone for gibbon ape leukemia virus. J. Virol. ; 71: 7619-7622, 1997.
• Plath, T.; Knudsen, C.R.; Bilgin, N.; Lindschau, C.; Erdmann, V.A.; Lippmann, C.: From genes to functional gene products during ageing. In: NATO ASI Series, Cell Biology . Heilmeyer, L. (Ed.). Springer-Verlag: 231-234, 1997.
• Qin, Q.-P.; Christiansen, M.; Oxvig, C.; Petterson, K.; Sottrup-Jensen, L.; Koch, C.; Nørgaard-Pedersen, B.: Double-monoclonal immunofluorometric assays for pregnancy- associated plasma protein-A/proeosinophil major basic protein (PAPP-A/proMBP) complex in first-trimester maternal serum screening for Down's syndrome. Clin. Chem. ; 43: 2323-2332, 1997.
• Rasmussen, J.O.; Waara, S.; Rasmussen, O.S.: Regeneration and analysis of interspecific asymmetric potato - Solanum ssp hybrid plants selected by micromanipulation or fluorescence-activated cell sorting (FACS). Theor. Appl. Genet. ; 95: 41-49, 1997.
• Rasmussen, J.T.; Berglund, L.; Petersen, T.E.: Structural characterization of bovine xanthine oxidoreductase from the milk fat globule membrane. In: Flavins and Flavoproteins 1996 . Stevenson, K.J.; Massey, V.; Williams Jr., C.H. (Eds.). University of Calgary Press, ISBN 1-895176-94-8, Calgary, Canada: 847-850, 1997.
• Rasmussen, L.K.; Sørensen, E.S.; Petersen, T.E.; Nielsen, N.C.; Thomsen, J.K.: Characterization of phosphate sites in native ovine, caprine, and bovine casein micelles and their caseinomacropeptides: A solid-state phosphorus-31 nuclear magnetic resonance and sequence and mass spectrometric study. J. Dairy Sci. ; 80: 607-614, 1997.
• Rattenborg, T.; Nautrup Pedersen, G.; Clark, B.F.C.; Knudsen, C.R.: Contribution of Arg288 of E. coli elongation factor Tu to translational functionality. Eur.J. Biochem. ; 249-2: 408-414, 1997.
• Rowatt, E.; Sørensen, E.S.; Triffit, J.; Viess, A.; Williams, R.J.: An examination of the binding of aluminum to protein and mineral components of bone and teeth. J. Inorg. Biochem. ; 68: 235-238, 1997.
• Sandal, N.N.; Salentijn, E.M.J.; Kleine, M.; Cai, D.; de Reuver, M.A.; van Druten, M.; de Bock, T.S.M.; Lange, W.; Steen, P.; Jung, C.; Marcker, K.; Stiekema, W.J.; Klein-Langhorst, R.M.: Backcrossing of nematode-resistant sugar beet: a scond nematode resistant gene at the locus containing Hs1pro1? Mol. Breeding ; 3: 471-480, 1997.
• Sanghera, D.K.; Kristensen, T.; Hamman, R.F.; Kamboh, M.I.: Molecular basis of the apolipoprotein H (β ₂ -glycoprotein I) protein polymorphism. Hum. Genet. ; 100: 57-62, 1997.
• Sperling-Petersen, H.U.; Kildsgaard, J.; Palacios, J.M.; Steffensen, S.; Egebjerg, J.; Mortensen, K.M.: A six-domain structural model for Escherichia coli translation initiation factor IF2. Characterisation of twelve surface epitopes. 17th International tRNA workshop, maj 1997. Ciba, Japan, 1997.
• Sperling-Petersen, H.U.; Kildsgaard, J.; Palacios, J.M.; Steffensen, S.; Egebjerg, J.;. Mortensen, K.K.: A functional and structural model for Escherichia coli translation initiation factor IF2. FASEB J ; 11, A1404: 3206, 1997.
• Steffensen, S.; Fage-Larsen, J.; Mortensen K.K.; Sperling-Petersen, H.U.: Comparison of the IF2 sequence from E.coli with four new enterobacterial IF2 sequences. Study of the internal initiation phenomenon. Human Capital and Mobility Network on Molecular Mechanism of Initiation of Prokaryptic Translation and Molecular Mechanism of Elongation Factor Tu Joint Annual Meeting, maj 1997. Island of Spetses, Greece, 1997.
• Steffensen, S.A. de A.; Poulsen, A.B.; Mortensen, K.; Sperling-Petersen, H.U.: E. coli translation initiation factor IF2 - an extremely conserved protein. Comparative sequence analysis of the infB gene in clinical isolates of E. coli . FEBS Lett. ; 419: 281-284, 1997.
• Sørensen, C.B.; Berglund, L.; Petersen, T.E.: Cloning of the murine tetranectin gene and 5'-flanking region. Gene ; 201: 199-202, 1997.
• Sørensen, E.S.; Rasmussen, L.K.; Møller, L.; Petersen, T.E.: Localization and multimeric nature of component PP3 in bovine milk. Purification and characterization of PP3 from caprine and ovine milk. J. Dairy Sci. ; 80: 3176-3181, 1997.
• Thomsen, C.; Sørensen, P.O.; Egebjerg, J.: 1-(3-(9H-carbazol-9-yl)-1-propyl)-4-(2-methoxyphenyl)-4-piperidinol, a novel subtype selective inhibitor of the mouse GABA-transporter, GAT2. Br.J. Pharmacol. ; 120: 983-985, 1997.
• Thykjær, T.; Danielsen, D.; She, Q.; Stougaard, J.: Organization and expression of genes in the genomic region surrounding the glutamine synthetase Gln1 gene from Lotus japonicus . Mol. Gen. Genet. ; 255: 628-636, 1997.
• Thykjær, T.; Finneman, J.; Schauser, L.; Christensen, L.; Poulsen, C.; Stougaard, J.: Gene targeting approaches using positive-negative selection and large flanking regions. Plant Mol. Biol. ; 35: 523-530, 1997.
• Turpaev, K.; Hartmann, R.; Kisselev, L.; Justesen, J.: Ap3A and Ap4A are primers for oligoadenylate synthesis catalyzed by interferon-inducible 2-5A synthetase. FEBS Lett. ; 408: 177-181, 1997.
• Østergaard, M.; Pedersen, L.; Schmidt, J.; Luz, A.; Lovmand, J.; Erfle, V.; Pedersen, F.S.; Strauss, P.G.: Mapping of a major osteomagenic determinant of murine leukemia virus RFB-14 to non-LTR sequences. J. Virol. ; 71: 645-649, 1997.